Isolation and characterization of a fructosyltransferase gene from Streptococcus mutans GS-5
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Isolation and characterization of a fructosyltransferase gene from Streptococcus mutans GS-5.
A fructosyltransferase (FTF) gene from Streptococcus mutans GS-5 has been isolated from a lambda L47.1 clone bank. The gene was contained on an 11.7-kilobase GS-5 DNA fragment and was initially subcloned into plasmid pACYC184 as a 5.4-kilobase HindIII fragment. However, further analysis revealed that transcription of the FTF gene was initiated at the P1 promoter contained on the vector. It was ...
متن کاملGenetic regulation of fructosyltransferase in Streptococcus mutans.
Streptococcus mutans possesses several extracellular sucrose-metabolizing enzymes which have been implicated as important virulence factors in dental caries. This study was initiated to investigate the genetic regulation of one of these enzymes, the extracellular fructosyltransferase (Ftf). Fusions were constructed with the region upstream of the S. mutans GS5 Ftf gene (ftf) and a promoterless ...
متن کامل[Isolation and characterization of Streptococcus mutans].
s), vol. 2. Information Retrieval. Inc.. Washington, D.C. 23. Pollock, J. J., L. I. Katona, H. Goodman, M. I. Cho, and V. J. Iacono. 1981. Bacteriolysis of Streptococcus IoluttlIos BHT by lysozyme and inorganic anions normally present in human saliva. Arch. Oral Biol. 26:711-716. 24. Reider, J. L., and F. L. Macrina. 1977. Plasmid DNA isolation in Streptococcuts ot1iut(utns: glycine-enhanced ce...
متن کاملIdentification of the Streptococcus mutans frp gene as a potential regulator of fructosyltransferase expression.
Four putative open reading frames (ORFs) were previously identified in the regions flanking the Streptococcus mutans GS-5 fructosyltransferase (FTF) gene. One of these, ORF 3, appeared to code for a low-molecular-mass protein containing amino acid sequences sharing homology with several Gram-positive bacterial DNA-binding proteins and it was suggested that the ORF 3 gene product might be an FTF...
متن کاملIsolation, characterization and sequence analysis of the scrK gene encoding fructokinase of Streptococcus mutans.
A gene encoding an ATP-dependent fructokinase from Streptococcus mutans GS-5 was identified within a 2 kb DNA fragment immediately downstream from the scrA gene. The gene cloned in Escherichia coli also expressed mannokinase activity. Insertional inactivation of this gene in S. mutans markedly decreased both fructokinase and mannokinase activities. Nucleotide sequence analysis of the 2 kb fragm...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1986
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.52.1.166-170.1986